Cell Line Engineering & Assay Development

WuXi AppTec offers comprehensive cell line development capabilities that start when clients provide the cDNA and transfection vector for subsequent molecular cloning. After transfection into a client-specified protein expression cell line (e.g., CHO, NSO), WuXi AppTec will work with the client to determine the appropriate number of clones to analyze — based on the client's time and budget restrictions — in order to find high-expressing product clones.

Identification and selection of high-producing stable clones can be subjected to additional rounds of subcloning or further analysis such as short (10 generations) or long (>30 generations) stability studies and product analysis/characterization. The top selected clone(s) can be transitioned to WuXi AppTec's cell culture process development team for media and cell culture optimization studies as well as GMP cell banking and cell line characterization activities.

For cell line development activities involving monoclonal antibodies (MAb) WuXi AppTec also offers hybridoma development services and molecular cloning of the light and heavy chains (including humanization activities) into a client-specified mammalian cell culture expression system (e.g., CHO).