Immunology and Inflammation

WuXi Immunology and Inflammation provides a variety of in vitro assays and in vivo models in the field of immunology/inflammation. We target major inflammatory pathways including Th1, Th2, and Th17-mediated disease models. We customize study designs and provide high quality integrated service for efficacy evaluation and mechanism of action research.

Scope

  • In vitro and ex vivo assays

    We offer services for in vitro assay development and lymphocyte studies using a combination of different techniques including ELISA, ELISPOT, flow cytometric analysis, RT-PCR, Western-blot.

    • T-cell Activation & Functions
      • T cell proliferation/activation assays
      • Th1/ Th2/ Th17/ Treg skewing assays
      • Cytokine assays
    • B-cell Activation & Functions
      • B cell activation/proliferation via TLR, CD40, BCR pathways
      • Antibody/isotype analysis
  • In vivo models

  • Autoimmune Arthritis

    Collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AIA) have been extensively used as animal models for rheumatoid arthritis (RA) in human. Compared to CIA model, AIA is induced by substances that do not contain a defined self antigen. In this respect, the model resembles spontaneous arthritic diseases in human and can be used to study basic mechanisms of how external triggering may lead to undesired self recognition.

    Experimental Autoimmune Encephalomyelitis (EAE)

    Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis (MS) in human. EAE and MS are inflammatory demyelinating diseases of the central nervous system (CNS). EAE displays different clinical patterns in different strains of animals, with a chronic-progressive clinical course in C57BL/6 mice, a relapsing-remitting course in SJL mice, and an acute onset and spontaneous recovery course in Lewis rats. EAE model provides a powerful model for studying the pathogenesis and immune regulation of CD4+ Th1/Th17-mediated tissue inflammation.

    OVA-induced Allergic Asthma

    Asthma is characterized by airway hyperreactivity (AHR), infiltration of eosinophils and elevated levels of IgE and Th2 cytokines (e.g., IL-4, IL-5 and IL-13). Murine model of allergen-induced asthma is widely used to simulate human AHR. BALB/c mice are a most often used strain in which both AHR and IgE responses are easily inducible.

    Psoriasis

    Psoriasis is a chronic inflammatory skin disease characterized by erythema, scale and skin thickening. Treated topically with the toll-like receptor 7 agonist imiquimod activates the innate immune system followed by the bridging and induction of adaptive immunity, leads to psoriasis. The model not only can be used to examine drug efficacy but also provide insights of how the innate immune system plays its role in driving the autoimmune T cell cascade.

    Delayed Type Hypersensitivity (DTH)

    Oxazolone or dinitrofluorobenzene (DNFB) are contact-sensitizing agents that can easily penetrate intact skin and bind covalently as hapten to a variety of endogenous proteins. The cardinal feature of the induced DTH is ear skin swelling and thickening, caused by T cells, macrophages and related cytokines.